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祝贺中国医学科学院老师使用南京卡米洛ELISA试剂盒发表分文章获高分录??!

发布时间:2023-05-17   点击次数:118次
文献标题Identifcation and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium ofcinale
文献作者Yong-MeiXing、Ming-MingZhao、Li-ChengGuo、Bing Li1、Juan Chen、Shun XingGuo
期刊名称Scientific Reports影响因子4.996
使用产品植物钙和钙调素依赖型蛋白激酶家族(SnRKs CCaMK)ELISA试剂盒货号2Pl-KMLJ91921p

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Identifcation and expression of  DoCCaMK during Sebacina sp.  symbiosis of Dendrobium ofcinale


Yong-MeiXing1,4, Ming-MingZhao1,2,4, Li-ChengGuo2,3, Bing Li1, Juan Chen1?? & ShunXingGuo1??


Dendrobium ofcinale Kimura et Migo is a famous precious medicinal plant in China. Seed and seedling  were cultivated with the mycorrhizal fungus Sebacina sp. CCaMK was initially cloned from D. ofcinale based on a SSH cDNA library of symbiotically germinated seeds with Sebacina sp. Phylogenetic  analysis was performed among DoCCaMK and other CCaMKs. The particle bombardment technique  was used to visualize DoCCaMK-GFP. qRT-PCR and western blot analysis were conducted to determine  the tissue expression patterns of DoCCaMK with (SGS) and without (UGS) Sebacina sp. Furthermore,  the efect of KN-93 on CCaMK expression was also examined. Using NMT the net Ca2+ fuxes and the  CCaMK concentration were measured during D. ofcinale seed germination. DoCCaMK had the highest  homology with Lilium longiforum CCaMK. The DoCCaMK-GFP protein localized in the nucleus and cell  membrane. CCaMK expression was signifcantly upregulated after symbiosis with Sebacina sp. KN-93  could be used as an inhibitor of CCaMK to inhibit D. ofcinale seed germination. Ca2+ infux and the  concentration of the CCaMK in the SGS group was signifcantly more than that of the UGS group. The  characterization of CCaMK provides certain genetic evidence for the involvement of this gene during  seed germination and mycorrhizal cultivation in D. ofcinale.




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